Intracellular flow analysis of Caspase3 on Jurkat cells using 0.5 µg/ 10^6 cells of antibody (Clone: ABM11D3). Green represents isotype control; red represents anti-Caspase-3 antibody. Goat anti-mouse PE conjugate was used as secondary. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).